PC 3144 Kinetics of cr - Chymotrypsin - catalyzed Hydrolysis

نویسندگان

  • M. L. Bender
  • G. E. Clement
  • C. R. Gunter
چکیده

dr:Y fl96.?‘). \_ _-, .~ % 18.5 39.1 30.0 36.7 44.6 w sot 491 7. ZERNER, B., AND BENDER, M. L., J. Am. Chem. Sot., 86, 356 (1963). 8. JENCKS, W. P., J. Am. Chem. Sot., 80,458l (1958). 9. CAPLOW, M., AND JENCKS, W. P., J. Biol. Chem., 238, PC3140 (1963). 10. BENDEH,M. L., CLEMENT,G. E., GUNTER,~. R., AND KEZDY, F. J., J. Biol. Chem., 238, PC3143 (1963). 11. EPAND, R. M., AND WILSON, I. B., J. Biol. Chem., 238, PC3138 (1963).

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The application of insolubilized alpha-chymotrypsin to kinetic studies on the effect of aprotic dipolar organic solvents.

Insolubilized cr-chymotrypsin bound to porous glass gel has been prepared. The kinetic behavior of this fixed enzyme proved to be the same as that of the free enzyme. The effects of aprotic dipolar organic solvents on the kinetics of cr-chymotrypsin-catalyzed hydrolysis were successfully studied using this insolubilized enzyme at concentrations up to 95% v/v dioxane, taking advantage of the ina...

متن کامل

The Application of Insolubilized a-Chymotrypsin to Kinetic Studies on the Effect of Aprotic Dipolar Organic Solvents*

Insolubilized cr-chymotrypsin bound to porous glass gel has been prepared. The kinetic behavior of this fixed enzyme proved to be the same as that of the free enzyme. The effects of aprotic dipolar organic solvents on the kinetics of cr-chymotrypsin-catalyzed hydrolysis were successfully studied using this insolubilized enzyme at concentrations up to 95% v/v dioxane, taking advantage of the ina...

متن کامل

Kinetics of alpha-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants.

The kinetics of alpha-chymotrypsin (alpha-CT) catalyzed hydrolysis of 4-nitrophenyl acetate has been studied in aqueous solution of alkyldimethylethanolammonium bromide (cetyl, dodecyl, decyl) surfactants at concentrations below and above their critical micelle concentration. From Michaelis-Mcnten kinetics, the catalytic rate constant kcat and the Michaelis constant KM have been determined. The...

متن کامل

Investigations of the chymotrypsin-catalyzed hydrolysis of specific substrates. I. The pH dependence of the catalytic hydrolysis of N-acetyl-L-tryptophanamide by three forms of the enzyme at alkaline pH.

The pH dependence of the steady state kinetic parameters K,(app) and $,t of the a-, acetylated 6-, and ar-chymotrypsincatalyzed hydrolysis of the specific amide substrate N-acetylr.-tryptophanamide, has been investigated in the neutral and alkaline pH regions. A completely automatic technique for measuring the hydrolysis of amides was used. New results and important aspects of the chymotrypsin-...

متن کامل

The kinetics of the alpha-chymotrypsin-catalyzed hydrolysis of p-nitrophenyl acetate in organic solvent-water mixtures.

The or-chymotrypsin-catalyzed hydrolysis of p-nitrophenyl acetate has been studied at pH 7 and 25’ in isopropyl alcoholwater and dioxane-water mixtures. It was found that the initial reaction in which the enzyme is acylated follows second order kinetics rather than Michaelis-Menten kinetics as previously reported. The second order rate constant for this reaction is markedly decreased by additio...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2003